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Versatile multicolor nanodiamond probes for intracellular imaging and targeted labeling

Author：Kerem Bray, Leonard Cheung, Khondker Rufaka Hossain, Igor Aharonovich, Stella M. Valenzuela, Olga Shimoni   《Journal of Materials Chemistry B》

ABSTRACTS
We report on the sizable production of fluorescent nanodiamonds (FNDs) containing a near infrared (NIR) color center – namely the silicon vacancy (SiV) defect, and their first demonstration inside cells for bio-imaging. We further demonstrate a concept of multi-color bio-imaging using FNDs to investigate intercellular processes using two types of FNDs. Due to their specific spectral properties, SiV FNDs can be distinguished from common nitrogen-vacancy (NV) FNDs and show a distinct initial spreading throughout the cell interior. The reported results are the first demonstration of multi-color labeling with FNDs that are especially interesting for in vivo bio-imaging due to their stable fluorescence.

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trans-activating transcriptional activator (TAT, China Peptides Ltd, 1 mg mL ?1 )
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http://pubs.rsc.org/en/content/articlelanding/2018/tb/c8tb00508g/unauth#!divAbstract
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