文献作者：Cui, Yanan; Li, Suxin; Chen, Binlong; He, Bing; Yuan, Lan; Dai, Wenbing; Zhang, Hua; Wang, Xueqing; Zhang, Qiang 《Current Pharmaceutical Biotechnology》 ABSTRACTS Background: Among the many researches on cellular uptake and intracellular trafficking pathways of αvβ3-targeted nanomedicines, a large number of studies utilize serum-free medium to evaluate drug effects and cellular mechanisms in vitro whether the medium is serum free or not has not been paid much attention. Methods: The aim of this study was to assess the impact of serum on αvβ3-mediated endocytosis and intracellular trafficking pathways. cRGDfK conjugated with Alexa Fluor? 555 was used to recognize αvβ3 integrins specifically. Transferrin-Alexa Fluor? 488 conjugates were selected as the intracellular trafficking pathway markers by real-time confocal analysis method using confocal laser-scanning microscope. Results: The results showed that after internalization, cRGDfK showed ...
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