Importance of Terminal Amino Acid Residues to the Transport of Oligopeptides across the Caco-2 Cell Monolayer
文献作者：Long Ding, Liying Wang, Zhipeng Yu, Sitong Ma, Zhiyang Du, Ting Zhang, and Jingbo Liu 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》 ABSTRACTS The objective of this paper was to investigate the effects of terminal amino acids on the transport of oligopeptides across the Caco-2 cell monolayer. Ala-based tetra- and pentapeptides were designed, and the N- or C-terminal amino acid residues were replaced by different amino acids. The results showed that the oligopeptides had a wide range of transport permeability across the Caco-2 cell monolayer and could be divided into four categories: non-/poor permeability, low permeability, intermediate permeability, and good permeability. Tetrapeptides with N-terminal Leu, Pro, Ile, Cys, Met, and Val or C-terminal Val showed the highest permeability, with apparent permeability coefficient ( P app ) values over 10 × 10 –6 cm/s ( p < 0.05), suggesting that nonpolar hydrophobic aliphatic amino ...