跳至主要内容

Peptide cleavage induced assembly enables highly sensitive electrochemiluminescence detection of protease activity

文献作者：Meng Cheng, Jun Zhou, Xiaoming Zhou, Da Xing   《Sensors and Actuators B: Chemical》

ABSTRACTS
Proteases perform essential functions in a multitude of physiological processes and participate in many human diseases. Probing protease activity sensitively and accurately is critical for both basic research and clinical diagnosis. Herein, a novel optical biosensor for high-sensitive detection of protease was developed based on the specific protease cleavage of synthesized peptide substrate and subsequent assembly between exposed cysteine and Ru(bpy)32+-2-cyanobenzothiazole (Ru(bpy)32+-CBT). The assembly complexes can be enriched by streptavidin coated magnetic beads on work electrode for high-sensitive electrochemiluminescence (ECL) analysis. Using trypsase and caspase-3 as the examples, the limit of detection (LOD) of 8.4?×?10?4?U?mL?1 and 5?×?10?6?U?mL?1 were obtained respectively. This represents one of most sensitive protease assay reported. Current peptide cleavage induced assembly (PCIA) method can be easily extended to detect other proteases by changing peptide substrate sequence, shows its great potential as versatile biosensor in sensing techniques and clinical diagnosis.
    KEY WORDS
    Electrochemiluminescence
    Ru(bpy)32+-2-cyanobenzothiazole (Ru(bpy)32+-CBT)
    Peptide cleavage induced assembly
    Trypsase
    Caspase-3
    SCREENSHOT

    RELATED PRODUCTS
    Peptides were synthesized by ChinaPeptides Co., Ltd (Shanghai, China).
    CHAINING
    https://www.sciencedirect.com/science/article/pii/S0925400518302156
    标签：

    评论

    发表评论

    此博客中的热门博文

    CAS:85466-18-8|Thymopoietin II (32-35)|RKDV

    Contact： Liven Qian(钱叶华)-Custom Peptide Mobile: 13761298676（微信） Email：cps037@chinapeptides.net QQ：2880526724 ChinaPeptides Co., Ltd./强耀生物科技有限公司 Name Thymopoietin II (32-35) Code [85466-18-8] The alias Thymopoietin II (32-35) Sequence (single letter abbreviation) RKDV Sequence (three-letter abbreviation) H-Arg-Lys-Asp-Val-OH (trifluoroacetate salt) A basic description TP-4 and TP-3; shortest active thymopoietin II fragments described that exhibit potent immunoregulatory properties in vitro and in vivo. solubility The molecular weight 516.6 Chemical formula C21H40N8O7 The purity 80%，90%，95%，98%，99% Weight 1mg,5mg,10mg,50mg,100mg,1g Storage conditions Store at -20°C. Keep tightly closed. Store in a cool dry place. Number of Residues: 4 1-Letter Code: RKDV 3-Letter Code: Arg-Lys-Asp-Val Molecular weight (Mr): 516.6 g/mol Isoelectric point: 10.1 Net charge at pH 7.0: 1.0 Average hydrophilicity: 1.9 Ratio of hydrophilic residues / total number of residues...
    1 条评论
    阅读全文

    β-Amyloid (1-42), human|DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

    Contact： Liven Qian(钱叶华)-Custom Peptide Mobile: 13761298676（微信） Email：cps037@chinapeptides.net QQ：2880526724 ChinaPeptides Co., Ltd./强耀生物科技有限公司   04010011526 β-Amyloid (1-42), human DAEFRHDSGYEVHHQKLVFFAEDV GSNKGAIIGLMVGGVVIA Number of Residues: 42 1-Letter Code: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 3-Letter Code: Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala Molecular weight (Mr): 4513.12 g/mol Isoelectric point: 5.8 Net charge at pH 7.0: -1.7 Average hydrophilicity: -0.1 Ratio of hydrophilic residues / total number of residues: 31 %    Name beta-Amyloid (1-42), recombinant Code   The alias beta-Amyloid (1-42), recombinant Sequence (single letter abbreviation) DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA Sequence (three-letter abbreviation) H-Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-...
    发表评论
    阅读全文

    The therapeutic effect of anti-CD52 treatment in murine experimental autoimmune encephalomyelitis is associated with altered IL-33 and ST2 expression levels

    文献作者：Mark Barbour, Rachel Wood, Shehla U.Hridi, Chelsey Wilson, Grant McKay, Trevor J.Bushell, Hui-Rong Jiang   《Journal of Neuroimmunology》 ABSTRACTS Experimental autoimmune encephalomyelitis  (EAE) mice were administered with murine anti-CD52  antibody  to investigate its therapeutic effect and whether the treatment modulates IL-33 and ST2 expression. EAE severity and  central nervous system  (CNS) inflammation were reduced following the treatment, which was accompanied by peripheral  T and B lymphocyte  depletion and reduced production of various  cytokines  including IL-33, while sST2 was increased. In spinal cords of EAE mice, while the number of IL-33 +  cells remained unchanged, the extracellular level of IL-33 protein was significantly reduced in anti-CD52 antibody treated mice compared with controls. Furthermore the number of ST2 +  cells in the spinal cord of treated EAE mice was  downregulated ...
    发表评论
    阅读全文