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The therapeutic effect of anti-CD52 treatment in murine experimental autoimmune encephalomyelitis is associated with altered IL-33 and ST2 expression levels

    文献作者：Mark Barbour, Rachel Wood, Shehla U.Hridi, Chelsey Wilson, Grant McKay, Trevor J.Bushell, Hui-Rong Jiang   《Journal of Neuroimmunology》
    ABSTRACTS
    Experimental autoimmune encephalomyelitis (EAE) mice were administered with murine anti-CD52 antibody to investigate its therapeutic effect and whether the treatment modulates IL-33 and ST2 expression. EAE severity and central nervous system (CNS) inflammation were reduced following the treatment, which was accompanied by peripheral T and B lymphocyte depletion and reduced production of various cytokines including IL-33, while sST2 was increased. In spinal cords of EAE mice, while the number of IL-33+ cells remained unchanged, the extracellular level of IL-33 protein was significantly reduced in anti-CD52 antibody treated mice compared with controls. Furthermore the number of ST2+ cells in the spinal cord of treated EAE mice was downregulated due to decreased inflammation and immune cell infiltration in the CNS. These results suggest that treatment with anti-CD52 antibody differentially alters expression of IL-33 and ST2, both systemically and within the CNS, which may indicate IL-33/ST2 axis is involved in the action of the antibody in inhibiting EAE.

    Graphical abstract


    KEY WORDS
    Multiple sclerosis; Experimental autoimmune encephalomyelitis; CD52; IL-33; ST2.
    SCREENSHOT

    RELATED PRODUCTS
    MOG 35–55
    CHAINING
    https://www.sciencedirect.com/science/article/pii/S0165572817304575
    Number of Residues:21
    1-Letter Code:MEVGWYRSPFSRVVHLYRNGK
    3-Letter Code:Met-Glu-Val-Gly-Trp-Tyr-Arg-Ser-Pro-Phe-Ser-Arg-Val-Val-His-Leu-Tyr-Arg-Asn-Gly-Lys

    Molecular weight (Mr):2581.99 g/mol
    Isoelectric point:10.6
    Net charge at pH 7.0:3.1
    Average hydrophilicity:-0.1
    Ratio of hydrophilic residues / total number of residues:38 %


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