跳至主要内容

Development of Graphene Oxide Functionalized Cotton Fiber Based Solid Phase Extraction Combined with Liquid Chromatography-Fluorescence Detection for Determination of Trace Auxins in Plant Samples

Author：Qiulian Zeng, Yuji Ruan, Lingshun Sun, Fuyou Du, Lin Guo, Zhenfang Cheng, Guihua Ruan, Jianping Li   《Chromatographia》

ABSTRACTS
In this work, a convenient and sensitive solid phase extraction (SPE) method was developed based on graphene oxide functionalized cotton fiber (GO-CF), a SPE sorbent followed by liquid chromatography-fluorescence detection (LC-FLD) for determination of trace indole acetic acid, indole propionic acid, indole butyric acid and naphthalene acetic acid. GO-CF was synthesized via covalent bonding method on the surface of cotton fiber, and exhibited higher extraction capacity in comparison with that of cotton fiber. The conditions affecting the extraction efficiency, including sample pH, elution solvent, elution volume, extraction time, and sample volume, were optimized to obtain high extraction recoveries. Under optimal conditions, the calibration curves were linear over the concentration ranging from 2 to 200 ng mL?1 with correlation coefficients between 0.9987 and 0.9998 for all the analytes. The limits of detection were lower than 0.371 ng mL?1 for the four auxins. The intra- and inter-day relative standard deviations (RSDs) were less than 3.5 and 7.1%, respectively. The mean recoveries ranged from 73.5 to 105.4% with RSDs lower than 12.6%. The developed GO-CF–SPE–LC-FLD method has been successfully applied to the analysis of four auxins in real plant samples, including mung bean stalk, mung bean sprout, and tobacco leaf samples.

GRAPHICAL ABSTRACT



KEY WORDS
Graphene oxide Cotton fiber Sample preparation Solid phase extraction Auxin 
SCREENSHOT

RELATED PRODUCTS
Peptides
CHAINING
https://link.springer.com/article/10.1007/s10337-018-3518-0
标签：

评论

发表评论

此博客中的热门博文

The therapeutic effect of anti-CD52 treatment in murine experimental autoimmune encephalomyelitis is associated with altered IL-33 and ST2 expression levels

文献作者：Mark Barbour, Rachel Wood, Shehla U.Hridi, Chelsey Wilson, Grant McKay, Trevor J.Bushell, Hui-Rong Jiang   《Journal of Neuroimmunology》 ABSTRACTS Experimental autoimmune encephalomyelitis  (EAE) mice were administered with murine anti-CD52  antibody  to investigate its therapeutic effect and whether the treatment modulates IL-33 and ST2 expression. EAE severity and  central nervous system  (CNS) inflammation were reduced following the treatment, which was accompanied by peripheral  T and B lymphocyte  depletion and reduced production of various  cytokines  including IL-33, while sST2 was increased. In spinal cords of EAE mice, while the number of IL-33 +  cells remained unchanged, the extracellular level of IL-33 protein was significantly reduced in anti-CD52 antibody treated mice compared with controls. Furthermore the number of ST2 +  cells in the spinal cord of treated EAE mice was  downregulated ...
发表评论
阅读全文

Mutational analysis of the extracellular disulphide bridges of the atypical chemokine receptor ACKR3/CXCR7 uncovers multiple binding and activation modes for its chemokine and endogenous non-chemokine agonists

Author：Martyna Szpakowska, Max Meyrath, Nathan Reynders,  Manuel Counson, Julien Hanson, Jan Steyaert, Andy Chevigné     《Biochemical Pharmacology》 ABSTRACTS The atypical chemokine receptor ACKR3/CXCR7 plays crucial roles in numerous physiological processes but also in viral infection and cancer. ACKR3 shows strong propensity for activation and, unlike classical chemokine receptors, can respond to chemokines from both the CXC and CC families as well as to the endogenous peptides BAM22 and adrenomedullin. Moreover, despite belonging to the G protein coupled receptor family, its function appears to be mainly dependent on β-arrestin. ACKR3 has also been shown to continuously cycle between the plasma membrane and the endosomal compartments, suggesting a possible role as a scavenging receptor. So far, the molecular basis accounting for these atypical binding and signalling properties remains elusive. Noteworthy, ACKR3 extracellular domains bear three  di...
发表评论
阅读全文

CAS:85466-18-8|Thymopoietin II (32-35)|RKDV

Contact： Liven Qian(钱叶华)-Custom Peptide Mobile: 13761298676（微信） Email：cps037@chinapeptides.net QQ：2880526724 ChinaPeptides Co., Ltd./强耀生物科技有限公司 Name Thymopoietin II (32-35) Code [85466-18-8] The alias Thymopoietin II (32-35) Sequence (single letter abbreviation) RKDV Sequence (three-letter abbreviation) H-Arg-Lys-Asp-Val-OH (trifluoroacetate salt) A basic description TP-4 and TP-3; shortest active thymopoietin II fragments described that exhibit potent immunoregulatory properties in vitro and in vivo. solubility The molecular weight 516.6 Chemical formula C21H40N8O7 The purity 80%，90%，95%，98%，99% Weight 1mg,5mg,10mg,50mg,100mg,1g Storage conditions Store at -20°C. Keep tightly closed. Store in a cool dry place. Number of Residues: 4 1-Letter Code: RKDV 3-Letter Code: Arg-Lys-Asp-Val Molecular weight (Mr): 516.6 g/mol Isoelectric point: 10.1 Net charge at pH 7.0: 1.0 Average hydrophilicity: 1.9 Ratio of hydrophilic residues / total number of residues...
1 条评论
阅读全文