跳至主要内容

A neurotrophic peptide-functionalized self-assembling peptide nanofiber hydrogel enhances rat sciatic nerve regeneration

Author：Jiaju Lu, Xun Sun, Heyong Yin, Xuezhen Shen, Shuhui Yang, Yu Wang, Wenli JiangYue Sun, Lingyun Zhao, Xiaodan Sun, Shibi Lu, Antonios G. Mikos, Jiang Peng, Xiumei Wang   《Nano Research》

ABSTRACTS
Nerve guidance conduit (NGC) is a potential alternative to autologous nerve for peripheral nerve regeneration. A promising therapeutic strategy is to modify the nerve guidance conduit intraluminal microenvironment using physical and/or chemical guidance cues. In this study, a neurotrophic peptide-functionalized self-assembling peptide nanofiber hydrogel that could promote PC12 cell adhesion, proliferation, and neuronal differentiation in vitro was prefilled in the lumen of a hollow chitosan tube (hCST) to accelerate axonal regeneration in a rat sciatic nerve defect model. The functionalized self-assembling peptide was developed by introducing a neurotrophic peptide (RGI, RGIDKRHWNSQ) derived from brain-derived neurotrophic factor (BDNF) to the C-terminus of the self-assembling peptide RADA16-I (Ac-(RADA)4-CONH2). Morphological, histological, electrophysiological, and functional analyses demonstrated that the RGI-functionalized, self-assembling, peptide nanofiber hydrogel RAD/RGI could produce a neurotrophic microenvironment that markedly improved axonal regeneration with enhanced re-myelination and motor functional recovery.

KEY WORDS
peripheral nerve regeneration ;self-assembling peptide ;hydrogel ;neurotrophic peptide ;intraluminal microenvironment .
SCREENSHOT


RELATED PRODUCTS
The SAPs were custom-synthesized through solid phase synthesis by China Peptides (Shanghai, China).
CHAINING
https://link.springer.com/article/10.1007/s12274-018-2041-9
标签：

评论

发表评论

此博客中的热门博文

Targeting the leptin receptor: To evaluate therapeutic efficacy and anti-tumor effects of Doxil, in vitro and in vivo in mice bearing C26 colon carcinoma tumor

Author：Shahrzad Amiri Darban, Sara Nikoofal-Sahlabadi,  Nafise Amiri, Nafiseh Kiamanesh, Amin Mehrabian, Bamdad Zendehbad, Zahra Gholizadeh, Mahmoud Reza Jaafari     《Colloids and Surfaces B: Biointerfaces》 ABSTRACTS Leptin is an appetite regulatory hormone that is secreted into the blood circulation by the adipose tissue and it functions via its over expressed receptors (Ob-R) in a wide variety of cancers. In the present study, the function of a leptin-derived peptide (LP16, 91–110 of Leptin) was investigated as a targeting ligand to decorate PEGylated liposomal doxorubicin (PLD, Doxil ? ) surface and the anti-tumor activity and therapeutic efficacy of Doxil in C26 (Colon Carcinoma) tumor model were also evaluated. As a result of this, Doxil with different LP16 peptide density (25, 50, 100 and 200 peptide on the surface of each liposome) was successfully prepared and characterized. In vitro results showed significant enhanced cytotoxicity and cellular b...
发表评论
阅读全文

Deubiquitinating enzyme USP9X regulates cellular clock function by modulating the ubiquitination and degradation of a core circadian protein BMAL1

Author：Yang Zhang, Chunyan Duan, Jing Yang, Suping Chen,  Qing Liu, Liang Zhou, Zhengyun Huang, Ying Xu, Guoqiang Xu     《Biochemical Journal》 ABSTRACTS Living organisms on the earth maintain a roughly 24 h circadian rhythm, which is regulated by circadian clock genes as well as their protein products. Post-translational modifications of core clock proteins could affect the circadian behavior. Although ubiquitination of core clock proteins was studied extensively, the reverse process, deubiquitination, has only begun to unfold and the role of this regulation on circadian function is not completely understood. Here, we use affinity purification and mass spectrometry analysis to identify probable ubiquitin carboxyl-terminal hydrolase FAF-X (USP9X) as an interacting protein of the core clock protein aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL or BMAL1). Through biochemical experiments, we discover that USP9X reduces BMAL1 ubiquitina...
发表评论
阅读全文

Electrochemical deposition of dopamine–hyaluronic acid conjugates for anti-biofouling bioelectrodes

文献作者：Semin Kim, Yohan Jang, Lindy K. Jang, Sung Hyuk Sunwoo, Tae-il Kim, Seung-Woo Cho and Jae Young Lee     《Journal of Materials Chemistry B》 ABSTRACTS Bioelectrodes have been widely used to effectively mediate electrical signals with biological systems for various biomedical applications, such as biosensors and prosthetic probes. However, the electrical properties of bioelectrodes are frequently degraded in the biological milieu due to biofouling of bioelectrode surfaces. Hence, the development of simple and effective strategies for bioelectrode surface modification is important for the mitigation of biofouling. To this end, we electrochemically modify electrodes with dopamine-conjugated hyaluronic acid (DA–HA); the modified electrodes exhibit highly hydrophilic surfaces. In addition, the electrochemical impedance of the DA–HA-modified electrodes remains similar to those of bare electrodes. The DA–HA-modified electrodes showed reduced non-specific protein ...
发表评论
阅读全文