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The effects of motif net charge and amphiphilicity on the self-assembly of functionally designer RADA16-I peptides

文献作者：Dongni Wu, Shuangying Zhang, Yuyuan Zhao, Ningjian Ao, Seeram Ramakrishna and Liumin He   《Biomedical Materials》

ABSTRACTS
RADA16-I (Ac-(RADA)4-CONH2) is a widely investigated self-assembling peptide (SAP) in the biomedical field. It can undergo ordered self-assembly to form stable secondary structures, thereby further forming a nanofiber hydrogel. The modification of RADA16-I with functional peptide motifs has become a popular research topic. Researchers aim to exhibit particular biomedical signaling, and subsequently, further expand its applications. However, only a few fundamental reports are available on the influences of the peptide motifs on self-assembly mechanisms of designer functional RADA16-I SAPs. In this study, we designed RGD-modified RADA16-I SAPs with a series of net charges and amphiphilicities. The assembly/reassembly of these functionally designer SAPs was thoroughly studied using Raman spectroscopy, CD spectroscopy, and AFM. The nanofiber morphology and the secondary structure largely depended on the balance between the hydrophobic effects versus like-charge repulsions of the motifs, which should be to the focus in order to achieve a tailored nanostructure. Our study would contribute insight into considerations for sophisticated design of SAPs for biomedical applications.
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The isoelectric points and net charge at neutral pH of the designer SAPs were calculated according to the free peptide calculator from the website of China Peptides Co., Ltd .
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http://iopscience.iop.org/article/10.1088/1748-605X/aab2fd/meta
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