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Serum Free or Not: Two Distinct Recycling Mechanisms Mediated by Alpha v Beta 3 Integrin

文献作者：Cui, Yanan; Li, Suxin; Chen, Binlong; He, Bing; Yuan, Lan; Dai, Wenbing; Zhang, Hua; Wang, Xueqing; Zhang, Qiang   《Current Pharmaceutical Biotechnology》

ABSTRACTS
Background: Among the many researches on cellular uptake and intracellular trafficking pathways of αvβ3-targeted nanomedicines, a large number of studies utilize serum-free medium to evaluate drug effects and cellular mechanisms in vitro whether the medium is serum free or not has not been paid much attention. 

Methods: The aim of this study was to assess the impact of serum on αvβ3-mediated endocytosis and intracellular trafficking pathways. cRGDfK conjugated with Alexa Fluor? 555 was used to recognize αvβ3 integrins specifically. Transferrin-Alexa Fluor? 488 conjugates were selected as the intracellular trafficking pathway markers by real-time confocal analysis method using confocal laser-scanning microscope. 

Results: The results showed that after internalization, cRGDfK showed perfect colocalization with transferrin (Tfn) when the cells were cultured in serum-free medium, but manifested obvious separation from Tfn to recycle back to the plasma membrane when the cells were cultured in complete medium. cRGDfK travels two quite different pathways under different culture conditions. 

Conclusion: We strongly recommended that when the intracellular mechanisms or pharmacodynamics of αvβ3-targeted nanomedicines was investigated, serum free medium or medium with serum should be taken into consideration. We hope this paper will provide helpful suggestions for studies on αvβ3- targeted drug delivery system.
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The pentapeptide cRGDfK was purchased from China Peptides Co., Ltd (Shanghai, China) .
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http://www.ingentaconnect.com/contentone/ben/cpb/2017/00000018/00000008/art00013
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The therapeutic effect of anti-CD52 treatment in murine experimental autoimmune encephalomyelitis is associated with altered IL-33 and ST2 expression levels

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Mutational analysis of the extracellular disulphide bridges of the atypical chemokine receptor ACKR3/CXCR7 uncovers multiple binding and activation modes for its chemokine and endogenous non-chemokine agonists

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CAS:85466-18-8|Thymopoietin II (32-35)|RKDV

Contact： Liven Qian(钱叶华)-Custom Peptide Mobile: 13761298676（微信） Email：cps037@chinapeptides.net QQ：2880526724 ChinaPeptides Co., Ltd./强耀生物科技有限公司 Name Thymopoietin II (32-35) Code [85466-18-8] The alias Thymopoietin II (32-35) Sequence (single letter abbreviation) RKDV Sequence (three-letter abbreviation) H-Arg-Lys-Asp-Val-OH (trifluoroacetate salt) A basic description TP-4 and TP-3; shortest active thymopoietin II fragments described that exhibit potent immunoregulatory properties in vitro and in vivo. solubility The molecular weight 516.6 Chemical formula C21H40N8O7 The purity 80%，90%，95%，98%，99% Weight 1mg,5mg,10mg,50mg,100mg,1g Storage conditions Store at -20°C. Keep tightly closed. Store in a cool dry place. Number of Residues: 4 1-Letter Code: RKDV 3-Letter Code: Arg-Lys-Asp-Val Molecular weight (Mr): 516.6 g/mol Isoelectric point: 10.1 Net charge at pH 7.0: 1.0 Average hydrophilicity: 1.9 Ratio of hydrophilic residues / total number of residues...
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