Anti-microRNA targeting using peptide-based nanocomplexes to inhibit differentiation of human pancreatic stellate cells

文献作者：Jonas Schnittert, Praneeth R Kuninty, Tomasz F Bystry, Roland Brock, Gert Storm & Jai Prakash   《Future Medicine》

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ABSTRACTS

Aim: To develop novel peptide-based nanocomplexes (NCs) for delivery of anti-miRNA oligonucleotides to human-derived pancreatic stellate cells (hPSCs), precursors of cancer-associated fibroblasts. Materials & methods: NCs of anti-miRNA oligonucleotides and cell-penetrating peptides (different variants) were formed and characterized. The effects of anti-miR-199a delivery on hPSC differentiation and 3D heterospheroid formation were investigated. Results: Dimeric cell-penetrating peptide based NCs (NC-2) showed 130-fold higher uptake by hPSCs compared with monomer-based NCs (NC-1) and tenfold higher uptake compared with general fibroblasts and different pancreatic tumor cells. Interestingly, delivery of anti-miR-199a inhibited hPSC differentiation into cancer-associated fibroblasts and inhibited the size of 3D heterospheroids comprised of hPSCs and tumor cells. Conclusion: Our NCs present a highly efficient anti-miRNA delivery system to hPSCs to inhibit their protumorigenic activity.

GRAPHICAL ABSTRACT

In this study, we developed novel cell-penetrating peptide based nanocomplexes (～40 nm) to efficiently deliver anti-miRNA-199a to primary human pancreatic stellate cells (hPSCs). Our nanocomplexes significantly inhibited the differentiation of hPSCs into tumor-promoting human pancreatic tumor stromal myofibroblasts and reduced the growth of in vitro tumor heterospheroids composed of hPSCs and Panc-1 epithelial cancer cells.

KEY WORDS

• cancer-associated fibroblasts; cell-penetrating peptides; microRNA-199a; miRNA delivery; nanocomplexes; pancreatic cancer; tumor microenvironment.

SCREENSHOT

RELATED PRODUCTS

The monomeric form of CPP (VSRRRRRRGGRRRR) and dimeric CPP (RRRRGGRRRRRRSV-CSSC-VSRRRRRRGGRRRR) were custom-synthesized from ChinaPeptides (Shanghai, China). 

CHAINING

https://www.futuremedicine.com/doi/abs/10.2217/nnm-2017-0054

Number of Residues:	14
1-Letter Code:	VSRRRRRRGGRRRR
3-Letter Code:	Val-Ser-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Gly-Arg-Arg-Arg-Arg

Molecular weight (Mr):	1880.21 g/mol
Isoelectric point:	12.4
Net charge at pH 7.0:	10.0
Average hydrophilicity:	2.1
Ratio of hydrophilic residues / total number of residues:	79 %

Number of Residues:	32
1-Letter Code:	RRRRGGRRRRRRSVCSSCVSRRRRRRGGRRRR
3-Letter Code:	Arg-Arg-Arg-Arg-Gly-Gly-Arg-Arg-Arg-Arg-Arg-Arg-Ser-Val-Cys-Ser-Ser-Cys-Val-Ser-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Gly-Arg-Arg-Arg-Arg

Molecular weight (Mr):	4122.84 g/mol
Isoelectric point:	12.4
Net charge at pH 7.0:	19.9
Average hydrophilicity:	1.8
Ratio of hydrophilic residues / total number of residues:	75 %