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Anti-microRNA targeting using peptide-based nanocomplexes to inhibit differentiation of human pancreatic stellate cells

文献作者：Jonas Schnittert, Praneeth R Kuninty, Tomasz F Bystry, Roland Brock, Gert Storm & Jai Prakash   《Future Medicine》

ABSTRACTS
Aim: To develop novel peptide-based nanocomplexes (NCs) for delivery of anti-miRNA oligonucleotides to human-derived pancreatic stellate cells (hPSCs), precursors of cancer-associated fibroblasts. Materials & methods: NCs of anti-miRNA oligonucleotides and cell-penetrating peptides (different variants) were formed and characterized. The effects of anti-miR-199a delivery on hPSC differentiation and 3D heterospheroid formation were investigated. Results: Dimeric cell-penetrating peptide based NCs (NC-2) showed 130-fold higher uptake by hPSCs compared with monomer-based NCs (NC-1) and tenfold higher uptake compared with general fibroblasts and different pancreatic tumor cells. Interestingly, delivery of anti-miR-199a inhibited hPSC differentiation into cancer-associated fibroblasts and inhibited the size of 3D heterospheroids comprised of hPSCs and tumor cells. Conclusion: Our NCs present a highly efficient anti-miRNA delivery system to hPSCs to inhibit their protumorigenic activity.

GRAPHICAL ABSTRACT

In this study, we developed novel cell-penetrating peptide based nanocomplexes (～40 nm) to efficiently deliver anti-miRNA-199a to primary human pancreatic stellate cells (hPSCs). Our nanocomplexes significantly inhibited the differentiation of hPSCs into tumor-promoting human pancreatic tumor stromal myofibroblasts and reduced the growth of in vitro tumor heterospheroids composed of hPSCs and Panc-1 epithelial cancer cells.
KEY WORDS
SCREENSHOT

RELATED PRODUCTS
The monomeric form of CPP (VSRRRRRRGGRRRR) and dimeric CPP (RRRRGGRRRRRRSV-CSSC-VSRRRRRRGGRRRR) were custom-synthesized from ChinaPeptides (Shanghai, China). 
CHAINING
https://www.futuremedicine.com/doi/abs/10.2217/nnm-2017-0054
Number of Residues:14
1-Letter Code:VSRRRRRRGGRRRR
3-Letter Code:Val-Ser-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Gly-Arg-Arg-Arg-Arg

Molecular weight (Mr):1880.21 g/mol
Isoelectric point:12.4
Net charge at pH 7.0:10.0
Average hydrophilicity:2.1
Ratio of hydrophilic residues / total number of residues:79 %


Number of Residues:32
1-Letter Code:RRRRGGRRRRRRSVCSSCVSRRRRRRGGRRRR
3-Letter Code:Arg-Arg-Arg-Arg-Gly-Gly-Arg-Arg-Arg-Arg-Arg-Arg-Ser-Val-Cys-Ser-Ser-Cys-Val-Ser-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Gly-Arg-Arg-Arg-Arg

Molecular weight (Mr):4122.84 g/mol
Isoelectric point:12.4
Net charge at pH 7.0:19.9
Average hydrophilicity:1.8
Ratio of hydrophilic residues / total number of residues:75 %

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CAS:85466-18-8|Thymopoietin II (32-35)|RKDV

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β-Amyloid (1-42), human|DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

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The therapeutic effect of anti-CD52 treatment in murine experimental autoimmune encephalomyelitis is associated with altered IL-33 and ST2 expression levels

文献作者：Mark Barbour, Rachel Wood, Shehla U.Hridi, Chelsey Wilson, Grant McKay, Trevor J.Bushell, Hui-Rong Jiang   《Journal of Neuroimmunology》 ABSTRACTS Experimental autoimmune encephalomyelitis  (EAE) mice were administered with murine anti-CD52  antibody  to investigate its therapeutic effect and whether the treatment modulates IL-33 and ST2 expression. EAE severity and  central nervous system  (CNS) inflammation were reduced following the treatment, which was accompanied by peripheral  T and B lymphocyte  depletion and reduced production of various  cytokines  including IL-33, while sST2 was increased. In spinal cords of EAE mice, while the number of IL-33 +  cells remained unchanged, the extracellular level of IL-33 protein was significantly reduced in anti-CD52 antibody treated mice compared with controls. Furthermore the number of ST2 +  cells in the spinal cord of treated EAE mice was  downregulated ...
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