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An Exploration of the Calcium-Binding Mode of Egg White Peptide, Asp-His-Thr-Lys-Glu, and In Vitro Calcium Absorption Studies of Peptide–Calcium Complex

文献作者：Na Sun, Ziqi Jin, Dongmei Li, Hongjie Yin, and Songyi Lin   《J. Agric. Food Chem.》

ABSTRACTS

The binding mode between the pentapeptide (DHTKE) from egg white hydrolysates and calcium ions was elucidated upon its structural and thermodynamics characteristics. The present study demonstrated that the DHTKE peptide could spontaneously bind calcium with a 1:1 stoichiometry, and that the calcium-binding site corresponded to the carboxyl oxygen, amino nitrogen, and imidazole nitrogen atoms of the DHTKE peptide. Moreover, the effect of the DHTKE–calcium complex on improving the calcium absorption was investigated in vitro using Caco-2 cells. Results showed that the DHTKE–calcium complex could facilitate the calcium influx into the cytosol and further improve calcium absorption across Caco-2 cell monolayers by more than 7 times when compared to calcium-free control. This study facilitates the understanding about the binding mechanism between peptides and calcium ions as well as suggests a potential application of egg white peptides as nutraceuticals to improve calcium absorption.
KEY WORDS
SCREENSHOT

RELATED PRODUCTS
Asp-His-Thr-Lys-Glu (DHTKE), was synthesized in 98.85% purity by China Peptides Co., Ltd. (Shanghai, China).
CHAINING
https://pubs.acs.org/doi/abs/10.1021/acs.jafc.7b03705
Number of Residues:5
1-Letter Code:DHTKE
3-Letter Code:Asp-His-Thr-Lys-Glu

Molecular weight (Mr):628.64 g/mol
Isoelectric point:5.2
Net charge at pH 7.0:-0.9
Average hydrophilicity:1.6
Ratio of hydrophilic residues / total number of residues:60 %
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CAS:85466-18-8|Thymopoietin II (32-35)|RKDV

Contact： Liven Qian(钱叶华)-Custom Peptide Mobile: 13761298676（微信） Email：cps037@chinapeptides.net QQ：2880526724 ChinaPeptides Co., Ltd./强耀生物科技有限公司 Name Thymopoietin II (32-35) Code [85466-18-8] The alias Thymopoietin II (32-35) Sequence (single letter abbreviation) RKDV Sequence (three-letter abbreviation) H-Arg-Lys-Asp-Val-OH (trifluoroacetate salt) A basic description TP-4 and TP-3; shortest active thymopoietin II fragments described that exhibit potent immunoregulatory properties in vitro and in vivo. solubility The molecular weight 516.6 Chemical formula C21H40N8O7 The purity 80%，90%，95%，98%，99% Weight 1mg,5mg,10mg,50mg,100mg,1g Storage conditions Store at -20°C. Keep tightly closed. Store in a cool dry place. Number of Residues: 4 1-Letter Code: RKDV 3-Letter Code: Arg-Lys-Asp-Val Molecular weight (Mr): 516.6 g/mol Isoelectric point: 10.1 Net charge at pH 7.0: 1.0 Average hydrophilicity: 1.9 Ratio of hydrophilic residues / total number of residues...
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