ChinaPeptides-Liven Qian

Contact： Liven Qian(钱叶华)-Custom Peptide Mobile: 13761298676（微信） Email：cps037@chinapeptides.net QQ：2880526724 ChinaPeptides Co., Ltd./强耀生物科技有限公司 Name [D-Ala4]-Substance P (4-11) Code [81381-50-2] The alias [D-Ala4]-Substance P (4-11) Sequence (single letter abbreviation) aQQFFGLM-NH2 Sequence (three-letter abbreviation) D-Ala-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 A basic description solubility The molecular weight 940.14 Chemical formula C44H65N11O10S The purity 80%，90%，95%，98%，99% Weight 1mg,5mg,10mg,50mg,100mg,1g Storage conditions Store at -20°C. Keep tightly closed. Store in a cool dry place.

Author：Araceli Garcia-Ac, Sung VoDuy, Sébastien Sauvé,  Florina Moldovan, V. Gaëlle Roullin, Xavier Banquy     《Talanta》 ABSTRACTS A method to explore the stability of two  anti-inflammatory   peptides   in human synovial fluid (HSF) has been developed and validated using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The two peptides are BQ123 Cyclo(- D -Trp- D -Asp- L -Pro- D -Val- L -Leu, Mw = 610.7) and R-954 (AcOrn[Oic 2 , (αMe)Phe 5 , DβNal 7 , Ile 8 ]desArg 9 -bradykinin, Mw =?1194.4). Human synovial fluid samples were analyzed after a  protein precipitation   step with  acetonitrile   and dilution with mobile phase. DMSO was used as anti-adsorptive agent. We used an octyl  silane   column with  formic acid   (0.1%, v/v) in water as the aqueous mobile phase and acetonitrile isopropanol-formic acid (20:80, 0.1?v/v) as the organic mobile phase and 0.7?mL/min flow rate. The...

Author：Ru-Tao Liu, Peng Zhang, Chun-Lin Yang, Yu Pang,  Min Zhang, Na Zhang, Long-Tao Yue, Xiao-Li Li, Heng Li, Rui-Sheng Duan     《Journal of Neuroimmunology》 ABSTRACTS Accumulating evidence shows that the  immunoproteasome   participates in the immune response, beyond its initial role in the  protein degradation . Here, we tested the effects of the selective immunoproteasome inhibitor, ONX-0914, on experimental  autoimmune   myasthenia gravis   (EAMG). We found that ONX-0914 ameliorated the severity of ongoing EAMG by reducing the  autoantibody   affinity, accompanied with decreased Tfh cells and  antigen presenting cells . Also it reduced the percentage of  Th17 cells   and inhibited the secretion of  IL-17 . Our data indicated ONX-0914 may bring benefit for MG therapy. Graphical abstract KEY WORDS ONX-0914;  Immunoproteasome; Experimental autoimmune myasthenia gravis; Humora...

Author：Ying Wang, Yiqun Jiang, Yongguang Yin, Jiyun Liu,  Long Ding, Jingbo Liu, Ting Zhang     《The Protein Journal》 ABSTRACTS Angiotensin I-converting enzyme (ACE, EC.3.4.15.1) inhibitory peptide is an efficacious therapy for hypertension. In this study, four dipeptides, TY, FD, FL and FG, were identified from the desalted fraction of bovine hemoglobin hydrolysate, obtained by in vitro simulated gastrointestinal digestion, via liquid chromatography-tandem mass spectrometry (LC-MS/MS). The IC 50   value of TY and FL are 96.43?±?6.17 and 290.66?±?57.92  μM, respectively. The result of molecular docking indicated that TY occupied the ACE subsite S1 and S1′ with a lowest estimated binding energy of ?9.96  Kcal/mol, while FL occupied the subsite S5 with a lowest estimated binding energy of ?9.37  Kcal/mol. The subsite S1′ and S2′ are closer to the ACE active center (Zn 2+ ) than S5, and the lowest estimated binding energy of TY is lower ...

Author：Liang Liu, Qingqing Xu, Shuai Hao & Yun Chen     《Scientific Reports》 ABSTRACTS MicroRNAs (miRNAs) play a vital role in regulating gene expression and are associated with a variety of cancers, including breast cancer. Their distorted and unique expression is a potential marker in clinical diagnoses and prognoses. Thus, accurate determination of miRNA expression levels is a prerequisite for their applications. However, the assays currently available for miRNA detection typically require pre-enrichment, amplification and labeling steps, and most of the assays are only semi-quantitative. Therefore, we developed a quasi-direct liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based targeted proteomics approach to quantify target miRNA by innovatively converting the miRNA signal into the mass response of a reporter peptide  via  a covalently immobilized DNA-peptide probe. Specifically, the probe containing the targeted proteomics-...

Author：Junjian Chen, Yuchen Zhu, Yancheng Song, Lin Wang,  Jiezhao Zhan, Jingcai He, Jian Zheng, Chunting Zhong, Xuetao Shi, Sa Liu, Li Ren and Yingjun Wang     《Journal of Materials Chemistry B》 ABSTRACTS Antimicrobial peptides (AMPs) are a broad prospect for clinical application against bacterial infections of biomaterials. However, a bottleneck exists as there is a lack of simple technology to prepare AMPs on biomaterials with sufficient activity, as the activity of AMP is dependent on the correct orientation on the biomaterial. In the present study, based on the conventional AMP (Tet213: KRWWKWWRRC) and surface binding peptide (SKHKGGKHKGGKHKG), we designed an Anchor-AMP that could be directly assembled onto the surface of the biomaterial and also showed excellent antimicrobial activity. By characterizing the surface using a quartz crystal microbalance with dissipation (QCM-D), contact angle, atom force microscopy (AFM) and X-ray photoelectron spectr...

Author：Han-jie Gu, Qing-lei Sun, Shuai Jiang, Jian Zhang,  Li Sun     《Developmental & Comparative Immunology》 ABSTRACTS Anti-lipopolysaccharide factor (ALF) is a type of  antimicrobial peptides   (AMPs) with a vital role in antimicrobial defense. Although a large amount of ALFs have been identified from neritic and fresh water crustacean species, no functional investigation of ALFs from deep-sea animals have been documented. In the present study, we characterized the immune function of an ALF molecule (named RspALF1) from the shrimp  Rimicaris   sp. residing in the deep-sea hydrothermal vent in Desmos, Manus Basin. RspALF1 shares 51.5%–62.4% overall sequence identities with known shrimp ALFs and contains the conserved LPS  binding domain   (LBD). Both  recombinant   RspALF1 (rRspALF1) and the LBD-derived  peptide   (ALF1P1) bound to the cell wall components of  Gram-negative   and  G...