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Individual and Synergistic Antioxidant Effects of Dipeptides in In Vitro Antioxidant Evaluation Systems

文献作者：Zhiyang Du, Jingbo Liu, Deju Zhang, Long Ding, Yizhen Wang, Dawei Tan, Ting Zhang   《International Journal of Peptide Research and Therapeutics》
ABSTRACTS
This study aimed to investigate the individual and synergistic antioxidant effects of dipeptides in vitro. Based on a study of amino acid antioxidant activities, 36 dipeptides were chosen. All their individual antioxidant effects were tested in in vitro antioxidant evaluation systems, including the DPPH, ABTS, ORAC, and FRAP assays. Based on the results of individual antioxidant effects of these dipeptides, some of them were chosen to study their synergistic antioxidant effects in the four antioxidant assays. The results of the DPPH assay showed that dipeptides containing Cys exhibited radical scavenging activity. The synergistic antioxidant assay indicated that LC and CH, LC and CN, LC and CE, CH and CN, CH and CE, and CN and CE have significant synergistic effects (p?<?0.01) in the DPPH assay. Moreover, the results of the ABTS assay showed that 17 dipeptides have ABTS radical scavenging activities and that all of them contain Cys, Trp, and Tyr residues. Their synergistic antioxidant assay showed that CR and CH, YR and YK, YR and YN, and WK and IW showed highly significant synergistic antioxidant effects (p?<?0.01). The results of the ORAC assay showed that 22 dipeptides possessed ORAC activities and that all of them have one or two Cys, His, Met, Trp, and Tyr residues. The synergistic effects of CH and HL, WK and WR, YK and WR, LC and FM, FM and CH, and WK and IW were significant (p?<?0.01). Finally, in the FRAP assay, six dipeptides showed ferric reducing antioxidant activities and all of them contained the Cys residue. The synergistic antioxidant effects of HV and CN, CK and CN, CH and CN, and CE and CN were also significant (p?<?0.01). These findings indicate that the individual and synergistic antioxidant effects of dipeptides are related to their constituent amino acids. These results may help study other individual and synergistic antioxidant effects of dipeptides.
KEY WORDS
Dipeptide Synergistic antioxidant DPPH ORAC ABTS FRAP
SCREENSHOT

RELATED PRODUCTS
All the peptides were synthesized by solid-phase proce- dures using Fmoc-protected amino acid synthetic methods by ChinaPeptides Co., Ltd. (Shanghai, China).
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https://link.springer.com/article/10.1007/s10989-018-9684-y
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CAS:85466-18-8|Thymopoietin II (32-35)|RKDV

Contact： Liven Qian(钱叶华)-Custom Peptide Mobile: 13761298676（微信） Email：cps037@chinapeptides.net QQ：2880526724 ChinaPeptides Co., Ltd./强耀生物科技有限公司 Name Thymopoietin II (32-35) Code [85466-18-8] The alias Thymopoietin II (32-35) Sequence (single letter abbreviation) RKDV Sequence (three-letter abbreviation) H-Arg-Lys-Asp-Val-OH (trifluoroacetate salt) A basic description TP-4 and TP-3; shortest active thymopoietin II fragments described that exhibit potent immunoregulatory properties in vitro and in vivo. solubility The molecular weight 516.6 Chemical formula C21H40N8O7 The purity 80%，90%，95%，98%，99% Weight 1mg,5mg,10mg,50mg,100mg,1g Storage conditions Store at -20°C. Keep tightly closed. Store in a cool dry place. Number of Residues: 4 1-Letter Code: RKDV 3-Letter Code: Arg-Lys-Asp-Val Molecular weight (Mr): 516.6 g/mol Isoelectric point: 10.1 Net charge at pH 7.0: 1.0 Average hydrophilicity: 1.9 Ratio of hydrophilic residues / total number of residues...
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