跳至主要内容

Individual and Synergistic Antioxidant Effects of Dipeptides in In Vitro Antioxidant Evaluation Systems

文献作者：Zhiyang Du, Jingbo Liu, Deju Zhang, Long Ding, Yizhen Wang, Dawei Tan, Ting Zhang   《International Journal of Peptide Research and Therapeutics》
ABSTRACTS
This study aimed to investigate the individual and synergistic antioxidant effects of dipeptides in vitro. Based on a study of amino acid antioxidant activities, 36 dipeptides were chosen. All their individual antioxidant effects were tested in in vitro antioxidant evaluation systems, including the DPPH, ABTS, ORAC, and FRAP assays. Based on the results of individual antioxidant effects of these dipeptides, some of them were chosen to study their synergistic antioxidant effects in the four antioxidant assays. The results of the DPPH assay showed that dipeptides containing Cys exhibited radical scavenging activity. The synergistic antioxidant assay indicated that LC and CH, LC and CN, LC and CE, CH and CN, CH and CE, and CN and CE have significant synergistic effects (p?<?0.01) in the DPPH assay. Moreover, the results of the ABTS assay showed that 17 dipeptides have ABTS radical scavenging activities and that all of them contain Cys, Trp, and Tyr residues. Their synergistic antioxidant assay showed that CR and CH, YR and YK, YR and YN, and WK and IW showed highly significant synergistic antioxidant effects (p?<?0.01). The results of the ORAC assay showed that 22 dipeptides possessed ORAC activities and that all of them have one or two Cys, His, Met, Trp, and Tyr residues. The synergistic effects of CH and HL, WK and WR, YK and WR, LC and FM, FM and CH, and WK and IW were significant (p?<?0.01). Finally, in the FRAP assay, six dipeptides showed ferric reducing antioxidant activities and all of them contained the Cys residue. The synergistic antioxidant effects of HV and CN, CK and CN, CH and CN, and CE and CN were also significant (p?<?0.01). These findings indicate that the individual and synergistic antioxidant effects of dipeptides are related to their constituent amino acids. These results may help study other individual and synergistic antioxidant effects of dipeptides.
KEY WORDS
Dipeptide Synergistic antioxidant DPPH ORAC ABTS FRAP
SCREENSHOT

RELATED PRODUCTS
All the peptides were synthesized by solid-phase proce- dures using Fmoc-protected amino acid synthetic methods by ChinaPeptides Co., Ltd. (Shanghai, China).
CHAINING
https://link.springer.com/article/10.1007/s10989-018-9684-y
标签：

评论

发表评论

此博客中的热门博文

The Catalytically Inactive Mutation of the Ubiquitin-Conjugating Enzyme CDC34 Affects its Stability and Cell Proliferation

Author：Xun Liu, Yang Zhang, Zhanhong Hu, Qian Li, Lu Yang,  Guoqiang Xu     《The Protein Journal》 ABSTRACTS The ubiquitin proteasome system (UPS) plays important roles in the regulation of protein stability, localization, and activity. A myriad of studies have focused on the functions of ubiquitin ligases E3s and deubiquitinating enzymes DUBs due to their specificity in the recognition of downstream substrates. However, the roles of the most ubiquitin-conjugating enzymes E2s are not completely understood except that they transport the activated ubiquitin and form E2–E3 protein complexes. Ubiquitin-conjugating enzyme CDC34 can promote the degradation of downstream targets through the UPS whereas its non-catalytic functions are still elusive. Here, we find that mutation of the catalytically active cysteine to serine (C93S) results in the reduced ubiquitination, increased stability, and attenuated degradation rate of CDC34. Through semi-quantitative proteom...
发表评论
阅读全文

Identification and Inhibitory Mechanism of Angiotensin I-Converting Enzyme Inhibitory Peptides Derived from Bovine Hemoglobin

Author：Ying Wang, Yiqun Jiang, Yongguang Yin, Jiyun Liu,  Long Ding, Jingbo Liu, Ting Zhang     《The Protein Journal》 ABSTRACTS Angiotensin I-converting enzyme (ACE, EC.3.4.15.1) inhibitory peptide is an efficacious therapy for hypertension. In this study, four dipeptides, TY, FD, FL and FG, were identified from the desalted fraction of bovine hemoglobin hydrolysate, obtained by in vitro simulated gastrointestinal digestion, via liquid chromatography-tandem mass spectrometry (LC-MS/MS). The IC 50   value of TY and FL are 96.43?±?6.17 and 290.66?±?57.92  μM, respectively. The result of molecular docking indicated that TY occupied the ACE subsite S1 and S1′ with a lowest estimated binding energy of ?9.96  Kcal/mol, while FL occupied the subsite S5 with a lowest estimated binding energy of ?9.37  Kcal/mol. The subsite S1′ and S2′ are closer to the ACE active center (Zn 2+ ) than S5, and the lowest estimated binding energy of TY is lower ...
发表评论
阅读全文

Preparation of an antimicrobial surface by direct assembly of antimicrobial peptide with its surface binding activity

Author：Junjian Chen, Yuchen Zhu, Yancheng Song, Lin Wang,  Jiezhao Zhan, Jingcai He, Jian Zheng, Chunting Zhong, Xuetao Shi, Sa Liu, Li Ren and Yingjun Wang     《Journal of Materials Chemistry B》 ABSTRACTS Antimicrobial peptides (AMPs) are a broad prospect for clinical application against bacterial infections of biomaterials. However, a bottleneck exists as there is a lack of simple technology to prepare AMPs on biomaterials with sufficient activity, as the activity of AMP is dependent on the correct orientation on the biomaterial. In the present study, based on the conventional AMP (Tet213: KRWWKWWRRC) and surface binding peptide (SKHKGGKHKGGKHKG), we designed an Anchor-AMP that could be directly assembled onto the surface of the biomaterial and also showed excellent antimicrobial activity. By characterizing the surface using a quartz crystal microbalance with dissipation (QCM-D), contact angle, atom force microscopy (AFM) and X-ray photoelectron spectr...
发表评论
阅读全文