跳至主要内容

Paper-based electrochemiluminescence sensor for highly sensitive detection of amyloid-β oligomerization: Toward potential diagnosis of Alzheimer's disease

文献作者：Hongxing Liu, Xiaoming Zhou, Qi Shen, Da Xing   《Theranostics》

ABSTRACTS
Development of a rapid and sensitive method for Aβ(1-42) aggregation detection is of great importance to overcome the limitations of conventional techniques. In this study, we developed a label-free paper-based electrochemiluminescence sensor for amyloid-β aggregation detection toward potential diagnosis of Alzheimer's disease (AD). The paper-based chip used in the system serves as a low-cost and disposable detection method. In this detection platform, the bonding of [Ru(phen)2dppz]2+ to Aβ(1-42) aggregates results in enhanced electrochemiluminescence due to the change in the polarity of the microenvironment when [Ru(phen)2dppz]2+intercalated into the β-sheets during oligomerization. The oligomerization process of Aβ(1-42) can be monitored in real time by the novel method, and as low as 100 pM equivalent monomer concentration of Aβ(1-42) could be detected simultaneously. In addition, the cerebrospinal fluid of transgenic AD model mice was tested by this method, which is highly consistent with genetic identification. In addition, we demonstrated that this detection platform could be a potential new method for the screening of Aβ(1-42) aggregation inhibitors, highlighting the practical application capacity of this platform. The platform is label free, low cost and sensitive. Therefore, the proposed platform holds great promise for the diagnosis of AD.
KEY WORDS
Aβ(1-42) aggregation, electrochemiluminescence, [Ru(phen)2dppz]2+, paper-based bipolar electrode, Alzheimer's disease
SCREENSHOT

RELATED PRODUCTS
Aβ(1-42), human (>95%) was purchased from ChinaPeptides (Shanghai, China).
CHAINING
http://thno.org/v08p2289.htm
Number of Residues:42
1-Letter Code:DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
3-Letter Code:Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala

Molecular weight (Mr):4514.1 g/mol
Isoelectric point:5.2
Net charge at pH 7.0:-2.7
Average hydrophilicity:-0.1
Ratio of hydrophilic residues / total number of residues:31 %

标签：

评论

发表评论

此博客中的热门博文

Micellar nanoformulation of lipophilized bortezomib: high drug loading, improved tolerability and targeted treatment of triple negative breast cancer

文献作者：Kaiqi Wu, Ru Cheng, Jian Zhang, Fenghua Meng,  Chao Denga and Zhiyuan Zhong     《Journal of Materials Chemistry B》 ABSTRACTS Bortezomib (BTZ) is the first proteasome inhibitor approved for the treatment of malignant tumors. The current clinical formulation, however, shows fast clearance, low tumor accumulation, and several side effects. Here, we report that micellar nanoformulation of lipophilized bortezomib achieves significantly enhanced drug loading, prolonged circulation time, improved tolerability and targeted treatment of triple negative breast cancer  in vivo . Lipophilized bortezomib, bortezomib-pinanediol (BP), was readily prepared in high yield. Interestingly, cRGD-targeted micelles based on poly(ethylene glycol)- b -poly(trimethylene carbonate- co -dithiolane trimethylene carbonate) achieved a high drug loading content of 8.05 wt% BTZ equiv. for BP, which was more than 8-fold higher than BTZ. BP-loaded cRGD-decorated micelles (B...
发表评论
阅读全文

The targeted antioxidant MitoQ causes mitochondrial swelling and depolarization in kidney tissue

Author：Esther M. Gottwald, Michael Duss, Milica Bugarski,  Dominik Haenni, Claus D. Schuh, Ehud M. Landau, Andrew M. Hall     《Physiological Reports》 ABSTRACTS Kidney proximal tubules (PTs) contain a high density of mitochondria, which are required to generate ATP to power solute transport. Mitochondrial dysfunction is implicated in the pathogenesis of numerous kidney diseases. Damaged mitochondria are thought to produce excess reactive oxygen species (ROS), which can lead to oxidative stress and activation of cell death pathways. MitoQ is a mitochondrial targeted antioxidant that has shown promise in preclinical models of renal diseases. However, recent studies in nonkidney cells have suggested that MitoQ might also have adverse effects. Here, using a live imaging approach, and both in  vitro and ex  vivo models, we show that MitoQ induces rapid swelling and depolarization of mitochondria in PT cells, but these effects were not observed with S...
发表评论
阅读全文

Near-infrared light-activated red-emitting upconverting nanoplatform for T1-weighted magnetic resonance imaging and photodynamic therapy

Author：Xiang-long Tang, Jun Wu, Ben-lan Lin, Sheng Cui, Hong-mei Liu, Ru-tong Yu, Xiao-dong Shen, Ting-wei Wang, Wei Xia     《Acta Biomaterialia》 ABSTRACTS Photodynamic therapy (PDT) has increasingly become an efficient and attractive cancer treatment modality based on reactive oxygen species (ROS) that can induce tumor death after  irradiation   with ultraviolet or visible light. Herein, to overcome the limited tissue penetration in traditional PDT, a novel near-infrared (NIR) light-activated NaScF4: 40% Yb, 2% Er@CaF2  upconversion  nanoparticle   (rUCNP) is successfully designed and synthesized.  Chlorin   e6, a  photosensitizer   and a chelating agent for Mn2+, is loaded into  human serum albumin   (HSA) that further conjugates onto rUCNPs. To increase the ability to target glioma tumor, an acyclic  Arg–Gly–Asp peptide   (cRGDyK) is linked to rUCNPs@HSA(Ce6-Mn). This nanoplatform enables e...
发表评论
阅读全文