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Inflammatory and biocompatibility evaluation of antimicrobial peptide GL13K immobilized onto titanium by silanization

文献作者：Lin Zhou, Zhaonan Lin, Jiamin Ding, Wenxiu Huang, Jiang Chen, Dong Wu   《Colloids and Surfaces B: Biointerfaces》

ABSTRACTS
The inflammatory reaction around the implant after implant placement is important not only for osseointegration but also for long-term implant survivals. In our study, GL13K, an antimicrobial peptide, was immobilized onto titanium surfaces to improve its anti-inflammatory properties. The method of silanization was used to immobilize the GL13K, which was confirmed by X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy, water contact angle measurement. DAPI fluorescence staining and Cell Counting Kit-8 (CCK-8) were used to measure the cell attachment and cell viability of the RAW264.7, which indicated a good cytocompatibility. Cellular morphology of RAW264.7 on modified surfaces showed less cell pseudopod. ELISA and qRT-PCR were performed to measure the inflammatory activity of the modified titanium surfaces. The secretion levels of pro-inflammatory cytokines interleukin (IL)-1β, tumor necrosis factor-alpha (TNF-α) and inducible nitric oxide synthase (iNOS) were downregulated at 12 h, 24 h, and 48 h, while the anti-inflammatory cytokines IL-10 and arginase were upregulated at 12 h, 24 h, and 48 h. All results indicate that the GL13K-coated titanium surfaces make the inflammatory process towards a less pro-inflammatory, which may promote the process of osseointegration.

Graphical abstract

KEY WORDS
Titanium; Antimicrobial peptide; Macrophage; Inflammatory; Silanization.
SCREENSHOT
RELATED PRODUCTS
 the AMP (GL13KGKIIKLKASLKLL CONH2, MW = 1424 g/mol) was synthesized by China Peptides Co., Ltd. (Suzhou, China)
CHAINING
https://www.sciencedirect.com/science/article/pii/S0927776517306471

Number of Residues:13
1-Letter Code:GKIIKLKASLKLL-NH2
3-Letter Code:Gly-Lys-Ile-Ile-Lys-Leu-Lys-Ala-Ser-Leu-Lys-Leu-Leu-NH2

Molecular weight (Mr):1423.89 g/mol
Isoelectric point:10.4
Net charge at pH 7.0:5.0
Average hydrophilicity:0.1
Ratio of hydrophilic residues / total number of residues:38 %


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