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Design of electrochemical biosensors with peptide probes as the receptors of targets and the inducers of gold nanoparticles assembly on electrode surface

文献作者：Ning Xia, Xin Wang, Jie Yu, Yangyang Wu, Shuchao Cheng, Yun Xing, Lin Liu   《Sensors and Actuators B: Chemical》

ABSTRACTS
We reported a general way to design electrochemical biosensors with peptide probes as the receptors of targets and the inducers of gold nanoparticles (AuNPs) assembly on electrode surface. To demonstrate the feasibility of our strategy, human chorionic gonadotropin (hCG) was first determined as a model analyte. Specifically, the hCG-binding peptide triggered the aggregation of AuNPs in solution; by modifying the electrode with the hCG-binding peptide, the peptide-induced AuNPs assembly was achieved on the electrode surface, resulting in the formation of a network of AuNPs and a significant fall of charge transfer resistance. The attachment of hCG onto the electrode surface through the probe-target interaction made the peptide lose its ability to trigger the formation of the AuNPs-based network architecture on electrode surface, thus leading to an increased charge transfer resistance. The electrochemical impedance technique allowed for the determination of hCG with a detection limit 0.6 mIU/mL. Furthermore, the method was used to the selective detection of amyloid-β oligomer (AβO, a reliable molecular biomarker and crucial target for the diagnosis and therapeutic intervention of Alzheimer's disease). Our result indicated that the AuNPs-based colorimetric assay can be developed into a corresponding electrochemical assay with significantly improving sensitivity and selectivity. Taking advantage of the simple principle and the unique physical and chemical properties of AuNPs, our work would be valuable for the design of novel electrochemical biosensors by marrying specific receptors.
KEY WORDS
Electrochemical biosensor; Human chorionic gonadotropin; Amyloid-β; Peptide aptamer; Gold nanoparticles; Colorimetric assay.
SCREENSHOT

RELATED PRODUCTS
Peptides used in this work were synthesized and purified by China Peptides Co., Ltd. (Shanghai, China) .
CHAINING
https://www.sciencedirect.com/science/article/pii/S0925400516313120
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CAS:85466-18-8|Thymopoietin II (32-35)|RKDV

Contact： Liven Qian(钱叶华)-Custom Peptide Mobile: 13761298676（微信） Email：cps037@chinapeptides.net QQ：2880526724 ChinaPeptides Co., Ltd./强耀生物科技有限公司 Name Thymopoietin II (32-35) Code [85466-18-8] The alias Thymopoietin II (32-35) Sequence (single letter abbreviation) RKDV Sequence (three-letter abbreviation) H-Arg-Lys-Asp-Val-OH (trifluoroacetate salt) A basic description TP-4 and TP-3; shortest active thymopoietin II fragments described that exhibit potent immunoregulatory properties in vitro and in vivo. solubility The molecular weight 516.6 Chemical formula C21H40N8O7 The purity 80%，90%，95%，98%，99% Weight 1mg,5mg,10mg,50mg,100mg,1g Storage conditions Store at -20°C. Keep tightly closed. Store in a cool dry place. Number of Residues: 4 1-Letter Code: RKDV 3-Letter Code: Arg-Lys-Asp-Val Molecular weight (Mr): 516.6 g/mol Isoelectric point: 10.1 Net charge at pH 7.0: 1.0 Average hydrophilicity: 1.9 Ratio of hydrophilic residues / total number of residues...
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